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IL4R (F6C4Z) Rabbit mAb (BSA and Azide Free) #37775
Filter:
- WB
- F
Western blot analysis of extracts from MC/9 and C2C12 cells using IL4R (F6C4Z) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Negative expression of IL4R protein in C2C12 cells is consistent with the predicted expression pattern. Data were generated using the standard formulation of this product.
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 75-80, 120 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
- Related Products
Product Information
Product Usage Information
This product is the carrier free version of product #56538. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #56538
For standard formulation of this product see product #56538
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
IL4R (F6C4Z) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total IL4R protein.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse IL4R protein.
Background
In the immune system, interleukin-4 (IL-4) controls B cell growth, survival, and maturation, in addition to proliferation and differentiation of the Th2 subset of T cells. IL-4 is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). It acts through the IL-4 receptor (IL4R), leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (3).
IL4R is a transmembrane ligand binding protein composed of a heterodimer alpha and gamma receptor complex (4,5). IL4R has been shown to bind both IL-4 and interleukin-13 (IL-13), regulating immunoglobulin E (IgE) and chemokine production during allergic inflammation (6). In addition to its role in regulating immune response, signaling through IL4R is also implicated in diseases such as asthma, allergy, and cancer, making it a potent target for various therapies (7-9). Increased IL4R expression has been observed in tumors (9,10), and several mutations in IL4R are associated with metastatic cancers, underscoring its importance in cancer progression and as a target for chemotherapy development (10,11).
IL4R is a transmembrane ligand binding protein composed of a heterodimer alpha and gamma receptor complex (4,5). IL4R has been shown to bind both IL-4 and interleukin-13 (IL-13), regulating immunoglobulin E (IgE) and chemokine production during allergic inflammation (6). In addition to its role in regulating immune response, signaling through IL4R is also implicated in diseases such as asthma, allergy, and cancer, making it a potent target for various therapies (7-9). Increased IL4R expression has been observed in tumors (9,10), and several mutations in IL4R are associated with metastatic cancers, underscoring its importance in cancer progression and as a target for chemotherapy development (10,11).
- Yokota, T. et al. (1986) Proc Natl Acad Sci U S A 83, 5894-8.
- Grabstein, K. et al. (1986) J Exp Med 163, 1405-14.
- Hou, J. et al. (1994) Science 265, 1701-6.
- Keegan, A.D. et al. (1994) Cell 76, 811-20.
- Naz, S. et al. (2019) Int J Biol Macromol 123, 239-245.
- Reece, P. et al. (2014) PLoS One 9, e100734.
- Massoud, A.H. et al. (2016) Nat Med 22, 1013-22.
- Aranda, C.J. et al. (2023) Allergy 78, 752-766.
- Viganò, E. et al. (2018) Blood 131, 2036-2046.
- Bankaitis, K.V. and Fingleton, B. (2015) Clin Exp Metastasis 32, 847-56.
- de Groot, A.E. et al. (2022) Neoplasia 32, 100830.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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