CD4 (RPA-T4) Mouse mAb (InTraSeq™ 3' Conjugate 3029) #87589
Filter:
- SCA
Human peripheral blood mononuclear cells (PBMCs) were processed with the InTraSeq™ 3’ protocol. The leftmost figure shows the Uniform Manifold Approximation and Projection (UMAP) of the annotated PBMC clusters. The figures display FeaturePlots of the CD4 RNA and CD4 (RPA-T4) Mouse mAb (InTraSeq™ 3' Conjugate 3029) protein expression levels. To generate similar plots, use the FeaturePlot command template from the Seurat computational package:
FeaturePlot(your_object, features = your_protein, max.cutoff = "q95", min.cutoff = "q5", order = TRUE) + scale_color_gradientn(colours = c("#22578B", "#73A9D2", "grey90", "#FDB393", "#b22222"))
FeaturePlot(your_object, features = your_protein, max.cutoff = "q95", min.cutoff = "q5", order = TRUE) + scale_color_gradientn(colours = c("#22578B", "#73A9D2", "grey90", "#FDB393", "#b22222"))
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 |
Application Key:
- SCA-Single Cell Analysis
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Storage
Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CD4 (RPA-T4) Mouse mAb (InTraSeq™ 3' Conjugate 3029) recognizes endogenous levels of total CD4 protein. This antibody detects an epitope within the extracellular domain.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with PHA-stimulated human peripheral blood mononuclear cells.
Background
Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part, and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and recruits the tyrosine kinase Lck, which is essential for T cell activation (1).
Pathways
Explore pathways related to this product.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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