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Phospho-CREB (Ser133) (87G3) Rabbit mAb (InTraSeq™ 3' Conjugate 3006) #95580
Filter:
- SCA
Cultured human peripheral blood mononuclear cells (PBMCs) treated with Cell Stimulation Cocktail (without Protein Transport Inhibitors) (500X) #29255 (30 min; Stimulated, left panels) or treated with Cell Stimulation Cocktail (without Protein Transport Inhibitors (500X) #29255 (30 min) then subsequently treated with Lambda Protein Phosphatase (40 min, 30°C, Stimulated + Phosphatase; right panels) were processed with the InTraSeq™ 3’ protocol. The T cell population was then selected and analyzed. The FeaturePlots in the top figures display the Phospho-CREB (Ser133) (87G3) Rabbit mAb (InTraSeq™ 3' Conjugate 3006) expression on the Uniform Manifold Approximation and Projection (UMAP). The FeaturePlots in the bottom figures display the CREB (48H2) Rabbit mAb (InTraSeq™ 3' Conjugate 3008) #41320 expression on the UMAP. To generate similar plots, use the FeaturePlot command template from the Seurat computational package: FeaturePlot(your_object, features = your_protein, max.cutoff = "q95", min.cutoff = "q5", order = TRUE) + scale_color_gradientn(colours = c("#22578B", "#73A9D2", "grey90", "#FDB393", "#b22222"))
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- SCA-Single Cell Analysis
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Related Products
Product Information
Storage
Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-CREB (Ser133) (87G3) Rabbit mAb (InTraSeq™ 3' Conjugate 3006) detects endogenous levels of CREB only when phosphorylated at serine 133. The antibody also detects the phosphorylated form of the CREB-related protein, ATF-1.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB.
Background
CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways, including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).
- Lonze, B.E. et al. (2002) Neuron 34, 371-85.
- Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
- Redmond, L. et al. (2002) Neuron 34, 999-1010.
- Dash, P.K. et al. (1990) Nature 345, 718-21.
- Yin, J.C. et al. (1994) Cell 79, 49-58.
- Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
- Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
- Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
- Tan, Y. et al. (1996) EMBO J 15, 4629-42.
Pathways
Explore pathways related to this product.
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For Research Use Only. Not for Use in Diagnostic Procedures.
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InTraSeq is a trademark of Cell Signaling Technology, Inc.
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