Product Information

Product Usage Information

Nocodazole is supplied as a lyophilized powder. For a 1 mg/ml stock, reconstitute the 10 mg in 10 ml DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 0.1-1 µg/ml for 12-48 hr. Soluble in DMSO.

Storage

Store lyophilized at room temperature or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 2 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight	301.3 g/mol
Purity	>98%
Molecular Formula	C₁₄H₁₁N₃O₃S
CAS	31430-18-9
Solubility	Soluble in DMSO at 10mg/ml.

Background

Nocodazole is an anti-neoplastic agent that reversibly interferes with the polymerization of microtubules (1). Widely-used as a cell cycle synchronizing agent in cell biology labs to induce mitotic arrest, investigators have demonstrated that high concentrations of nocodazole induce microtubule depolymerization, whereas low concentrations alter spindle microtubule dynamics, but microtubules do not depolymerize (2-4). Recent research studies have demonstrated nocodazole to be a common inhibitor of various cancer-related kinases, including: ABL, c-KIT, BRAF, MEK1, MEK2, and MET (5).

Alternate Names

ABL; anti-neoplastic; BRAF; c-KIT; MEK1; MEK2; MET; microtubule depolymerization; mitotic arrest; phospho histone; R 17934

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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Nocodazole #2190