F4/80 (BM8.1) Rat mAb (PerCP-Cy5.5® Conjugate) #88154
Filter:
- F
Flow cytometric analysis of live mouse peritoneal macrophages using F4/80 (BM8.1) Rat mAb (PerCP-Cy5.5® Conjugate) (right), compared to concentration-matched Rat (LTF-2) mAb IgG2b Isotype Control (PerCP-Cy5.5® Conjugate) #79201 (left),
costained with CD11b/ITGAM (M1/70) Rat mAb (FITC Conjugate) #24442.
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rat IgG2b kappa |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
- Related Products
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to PerCP-Cy5.5® and tested in-house for direct flow cytometric analysis in mouse cells.
Product Usage Information
For optimal flow cytometry results, we recommend 0.125μg of antibody per test.
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:160 |
| Flow Cytometry (Live) | 1:160 |
Storage
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
F4/80 (BM8.1) Rat mAb (PerCP-Cy5.5® Conjugate) recognizes endogenous levels of total F4/80 protein. This antibody detects an epitope within the extracellular domain.
Species Reactivity:
Mouse
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (1-3). Expression of F4/80 has also been observed in microglia and subset populations of dendritic cells (4).
BM8.1 is widely used together with antibodies to CD115 (c-Fms), CD11b, and CD11c to identify myeloid/macrophage cells by flow cytometry.
BM8.1 is widely used together with antibodies to CD115 (c-Fms), CD11b, and CD11c to identify myeloid/macrophage cells by flow cytometry.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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