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Helios (D8W4X) XP® Rabbit mAb (mFluor™ Red 780 Conjugate) #72658
Filter:
- F
Flow cytometric analysis of fixed/permeabilized THP-1 cells (blue, negative) and Jurkat cells (green, positive) using Helios (D8W4X) XP® Rabbit mAb (mFluor™ Red 780 Conjugate) (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (mFluor™ Red 780 Conjugate) #79088 (dashed lines).
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Related Products
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to mFluor™ Red 780 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated Helios (D8W4X) XP® Rabbit mAb #42427.
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
Helios (D8W4X) XP® Rabbit mAb (mFluor™ Red 780 Conjugate) recognizes endogenous levels of total Helios protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly73 of human Helios protein.
Background
Helios (Ikaros family zinc finger 2, IKZF2) is an Ikaros family transcription factor composed of several zinc fingers that mediate DNA binding and homodimerization or heterodimerization with other Ikaros family proteins (1,2). In the hematopoietic system, Helios expression is restricted to T cells and early hematopoietic progenitors (1,2). In regulatory T cells, expression of Helios contributes to an anergic phenotype by binding to the IL-2 promoter and suppressing IL-2 transcription (3). In addition, alteration of the corresponding Helios gene IKZF2 is one hallmark of low-hypodiploid acute lymphoblastic leukemia (4).
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
mFluor is a trademark of AAT Bioquest, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
mFluor is manufactured by AAT Bioquest, Inc.
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