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Myeloperoxidase (E1E7I) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #17646
Filter:
- IF
- F
Confocal immunofluorescent analysis of HL-60 cells (left, positive) and HeLa cells (right, negative) using Myeloperoxidase (E1E7I) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (red) and DAPI #4083 (blue).
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and tested in-house for direct flow cytometric and immunofluorescent analysis in human cells. This antibody conjugate is expected to exhibit the same cross-reactivity as the unconjugated Myeloperoxidase (E1E7I) XP® Rabbit mAb #14569.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:50 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
Myeloperoxidase (E1E7I) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total myeloperoxidase protein. This antibody recognizes the full-length and heavy chain subunits of human myeloperoxidase protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro734 of human myeloperoxidase protein.
Background
Myeloperoxidase (MPO) is a peroxidase enzyme that is part of the host defense system of polymorphonuclear leukocytes (reviewed in 1). The gene for MPO was cloned independently from several laboratories (2-5). A decrease in MPO expression was noticed upon differentiation of HL-60 cells (5). MPO catalyzes the reaction of hydrogen peroxide and chloride (or other halides) to produce hypochlorous acid and other potent antimicrobial oxidants. Knockout mice of MPO are impaired in clearing select microbial infections (6). Processing of mature MPO from an initial 80-90 kDa translation product involves insertion of a heme moiety, glycosylation, and proteolytic cleavage. The mature protein is a tetramer of two heavy chains (60 kDa) and two light chains (12 kDa). It is abundantly expressed in neutrophils and monocytes and secreted during their activation. Heightened MPO levels have been associated with tissue damage and a number of pathological conditions (1).
- Klebanoff, S.J. (2005) J Leukoc Biol 77, 598-625.
- Chang, K.S. et al. (1986) Blood 68, 1411-4.
- Yamada, M. et al. (1987) Arch Biochem Biophys 255, 147-55.
- Morishita, K. et al. (1987) J Biol Chem 262, 3844-51.
- Weil, S.C. et al. (1987) Proc Natl Acad Sci USA 84, 2057-61.
- Aratani, Y. et al. (2002) J Infect Dis 185, 1833-7.
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
XP is a registered trademark of Cell Signaling Technology, Inc.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
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