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FastScan™ Total Zap-70 ELISA Kit #23925

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ELISA Image 1: FastScan™ Total Zap-70 ELISA Kit
Figure 1. Treatment of Jurkat cells with H2O2 stimulates phosphorylation of Zap-70 at Tyr319 but does not affect the level of total Zap-70. The relationship between lysate protein concentration from untreated and H2O2-treated Jurkat cells and the absorbance at 450 nm using the FastScan™ Total Zap-70 ELISA Kit #23925 is shown in the upper figure. The corresponding western blots using Zap-70 antibody (left panel) and phospho-Zap-70 (Tyr319) antibody (right panel) are shown in the lower figure. After serum starvation, Jurkat cells were treated with 11 mM H2O2 for 3 minutes at 37°C and then lysed.
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To Purchase # 23925**

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H M
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Product Includes
  • Related Products
Product IncludesVolume (with Count)Solution Color
FastScan™ ELISA Microwell Strip Plate, 96 Well #532571 x 96 tests
Zap-70 Rabbit Capture mAb #324971 x 1 eaGreen (Lyophilized)
Zap-70 Mouse HRP-linked mAb #434121 x 1 eaRed (Lyophilized)
FastScan™ ELISA Capture Antibody Diluent #160761 x 3 mlGreen
FastScan™ ELISA HRP Antibody Diluent #281201 x 3 ml
TMB Substrate #70041 x 11 ml
STOP Solution #70021 x 11 ml
Sealing Tape #545031 x 1 ea
ELISA Wash Buffer (20X) #98011 x 25 ml
FastScan™ ELISA Cell Extraction Buffer (5X) #699051 x 10 ml
FastScan™ ELISA Cell Extraction Enhancer Solution (50X) #252431 x 1 ml
FastScan™ ELISA Kit #23925 Positive Control Type 2 #258421 x 1 ea

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The FastScan™ Total Zap-70 ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Zap-70. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with Zap-70 in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of Zap-70.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

The FastScan™ Total Zap-70 ELISA Kit detects endogenous levels of Zap-70 as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human, Mouse

Background

The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).

Pathways

Explore pathways related to this product.


Database Links

UniProt ID: P43403


Entrez-Gene Id: 7535


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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
FastScan™ ELISA is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patents 9,086,407, 9,261,500, and 9,476,874, foreign equivalents, and child patents deriving therefrom.
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