PathScan® RP ApoE Sandwich ELISA Kit #95652
- ELISA
To Purchase # 95652**
Important Ordering Details
Custom Ordering Details:If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.
Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.
Supporting Data
| REACTIVITY | M |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- M-Mouse
- Product Includes
- Related Products
| Product Includes | Volume | Solution Color |
|---|---|---|
| ApoE Rabbit mAb Coated Microwells #42119 | 96 tests | |
| ApoE Rabbit Detection mAb #33820 | 1 ea | Red (Lyophilized) |
| HRP Diluent #13515 | 5.5 ml | Red |
| TMB Substrate #7004 | 11 ml | |
| STOP Solution #7002 | 11 ml | |
| Sealing Tape #54503 | 2 ea | |
| ELISA Wash Buffer (20X) #9801 | 25 ml | |
| Cell Lysis Buffer (10X) #9803 | 15 ml |
Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.
The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.
Product Information
Product Description
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
Human ApoE has three isoforms: ApoE2, ApoE3, and ApoE4. These three isoforms differ in the combination of cysteine and arginine residues located at positions 130 and 176. The ApoE4 isoform contains arginine at both locations (3). Arginine 130 in ApoE4 allows for interaction between carboxy- and amino-terminal domains through orientation of arginine 61, leading to a preference for binding lower density lipoproteins, where ApoE2 and ApoE3 show preference for binding HDL. Mouse ApoE contains similar sequence to human ApoE4, with arginine present at equivalent positions to 130 and 176. However, the mouse sequence lacks arginine at critical position 61, which allows it to behave similarly to human ApoE3, including preferential binding to HDL (4).
限制使用
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