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  3. PathScan® RP Total CSF-1R/M-CSF-R Sandwich ELISA Kit

PathScan® RP Total CSF-1R/M-CSF-R Sandwich ELISA Kit #13032

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Figure 1. The PathScan® RP Total CSF-1R/M-CSF-R Sandwich ELISA Kit #13032 detects varying levels of CSF-1R/M-CSF-R protein from extracts of NKM-1, THP-1, SK-BR-3, HeLa, COS-7, RAW 264.7, C2C12, and PC-12 cells. The absorbance at 450 nm for a panel of cell extracts (0.2 mg/mL for each sample) using the PathScan® RP Total CSF-1R/M-CSF-R Sandwich ELISA Kit #13032 is shown in the upper figure. The corresponding western blot using CSF-1R/M-CSF-R antibody is shown in the lower figure.
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To Purchase # 13032**

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
CSF-1R/M-CSF-R Rabbit mAb Coated Microwells #5564996 tests
CSF-1R/M-CSF-R Rabbit Detection mAb #621741 eaRed (Lyophilized)
HRP Diluent #135155.5 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The rapid protocol (RP) PathScan® RP Total CSF-1R/M-CSF-R Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of CSF-1R/M-CSF-R protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with CSF-1R/M-CSF-R in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of CSF-1R/M-CSF-R. Learn more about all of your ELISA kit options here.

*Antibodies in kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

The PathScan® RP Total CSF-1R/M-CSF-R Sandwich ELISA Kit detects endogenous levels of CSF-1R/M-CSF-R protein. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

Macrophage-colony stimulating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the c-fms proto-oncogene. M-CSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage (1-3). Binding of M-CSF to its receptor induces receptor dimerization, activation, and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins (4). There are at least five major tyrosine autophosphorylation sites. Tyr723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated Tyr723 binds the p85 subunit of PI3 kinase as well as PLCγ2 (5). Phosphorylation of Tyr809 provides a docking site for Shc (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems (6). The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma (7) and breast cancer (8).

Pathways

Explore pathways related to this product.


Database Links

UniProt ID: P07333


Entrez-Gene Id: 1436


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For Research Use Only. Not for Use in Diagnostic Procedures.
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