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Histone H3 (D1H2) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3049) #77931
Filter:
- SCA
Cultured primary T cells (Sample 3) and human peripheral blood mononuclear cells (Sample 4) were processed in accordance with the InTraSeq™ 3’ protocol and analyzed using the Seurat computational package. Sample 3 was labeled with Histone H3 (D1H2) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3002) #68984, whereas Sample 4 was labeled with Histone H3 (D1H2) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3049). Then, both samples were pooled prior to the single cell experiment. The left figure shows the Uniform Manifold Approximation and Projection (UMAP) of the annotated Sample 3 and 4 clusters. The middle figure displays a FeaturePlot of the Histone H3 (D1H2) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3002) #68984 protein expression levels, and the right figure displays a FeaturePlot of the Histone H3 (D1H2) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3049) protein expression levels. To generate similar plots, use the FeaturePlot command template from the Seurat computational package:
FeaturePlot(your_object, features = your_protein, max.cutoff = "q95") + scale_color_gradientn(colours = c("grey90", "#b22222"))
FeaturePlot(your_object, features = your_protein, max.cutoff = "q95") + scale_color_gradientn(colours = c("grey90", "#b22222"))
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- SCA-Single Cell Analysis
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Related Products
Product Information
Storage
Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Histone H3 (D1H2) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3049) detects endogenous levels of total histone H3 protein, including isoforms H3.1, H3.2, and H3.3. This antibody conjugate also detects the histone H3 variant CENP-A; it does not cross-react with other core histones.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human histone H3 protein.
Background
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
- Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
- Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
- Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
- Cheung, P. et al. (2000) Cell 103, 263-71.
- Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
- Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
- Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
- Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
- Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
- Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
- Dai, J. et al. (2005) Genes Dev 19, 472-88.
Pathways
Explore pathways related to this product.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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