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NF-κB p65 (D14E12) XP ® Rabbit mAb (InTraSeq 3' Conjugate 3033) #76695

Filter:
  • SCA
SCA Image 1: NF-κB p65 (D14E12) XP ® Rabbit mAb (InTraSeq™ 3' Conjugate 3033)
Cultured human peripheral blood mononuclear cells (PBMCs) treated with Cell Stimulation Cocktail (without Protein Transport Inhibitors) (500X) #29255 (30 min; Stimulated, left panels) or treated with Cell Stimulation Cocktail (without Protein Transport Inhibitors) (500X) #29255 (30 min) then subsequently treated with Lambda Protein Phosphatase (40 min, 30°C, Stimulated + Phosphatase; right panels) were processed with the InTraSeq™ 3’ protocol. The T cell population was then selected and analyzed. The FeaturePlots in the top figures display the Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (InTraSeq™ 3' Conjugate 3034) #97410 expression on the UMAP. The FeaturePlots in the bottom figures display the NF-κB p65 (D14E12) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3033) expression on the UMAP. To generate similar plots, use the FeaturePlot command template from the Seurat computational package: FeaturePlot(your_object, features = your_protein, max.cutoff = "q95", min.cutoff = "q5", order = TRUE) + scale_color_gradientn(colours = c("#22578B", "#73A9D2", "grey90", "#FDB393", "#b22222"))

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Rabbit IgG
Application Key:
  • SCA-Single Cell Analysis 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Storage

Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

NF-κB p65 (D14E12) XP ® Rabbit mAb (InTraSeq™ 3' Conjugate 3033) recognizes endogenous levels of total NF-κB p65/RelA protein. It does not cross react with other NF-κB/Rel family members.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu498 of human NF-κB p65/RelA protein.

Background

Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).

Pathways

Explore pathways related to this product.


For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
10x Genomics, 10x, Feature Barcode, and Chromium are the trademarks or registered trademarks of 10x Genomics, Inc.
InTraSeq is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Subject to patents licensed from 10x Genomics, Inc. for use with single-cell (i.e., Chromium) 10x products.
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