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Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3015) #69098
Filter:
- SCA
Cultured human peripheral blood mononuclear cells (PBMCs) untreated (Cultured; left panels) or treated with Human IL-6 Recombinant Protein #48333, Human IL-2 Recombinant Protein #31058, Human IGF-I Recombinant Protein #29608, and Cell Stimulation Cocktail (without Protein Transport Inhibitors) (500X) #29255 (30 min, Stimulated; right panels) were processed in accordance with the InTraSeq™ 3’ protocol. The T cell population was then selected and analyzed. The FeaturePlots in the top figures display the Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3015) expression on the Uniform Manifold Approximation and Projection (UMAP). The FeaturePlots in the bottom figures display the Stat3 (124H6) Mouse mAb (InTraSeq™ 3' Conjugate 3022) #68474 expression on the UMAP. To generate similar plots, use the FeaturePlot command template from the Seurat computational package: FeaturePlot(your_object, features = your_protein, max.cutoff = "q99", min.cutoff = "q1", order = TRUE) + scale_color_gradientn(colours = c("#22578B", "#73A9D2", "grey90", "#FDB393", "#b22222"))
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- SCA-Single Cell Analysis
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Related Products
Product Information
Storage
Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3015) detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3.
Background
The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
- Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
- Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
- Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
- Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
- Bromberg, J.F. et al. (1999) Cell 98, 295-303.
- Darnell, J.E. et al. (1994) Science 264, 1415-21.
- Ihle, J.N. (1995) Nature 377, 591-4.
- Wen, Z. et al. (1995) Cell 82, 241-50.
- Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
- Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.
Pathways
Explore pathways related to this product.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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InTraSeq is a trademark of Cell Signaling Technology, Inc.
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