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GM-CSF Receptor α (E8Z3S) Rabbit mAb #69817
Filter:
- WB
- IHC
Western blot analysis of extracts from various human cell lines using GM-CSF Receptor α (E8Z3S) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Supporting Data
| REACTIVITY | |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70-80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:200 - 1:800 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #63107.
For a carrier free (BSA and azide free) version of this product see product #63107.
Protocol
Specificity / Sensitivity
GM-CSF Receptor α (E8Z3S) Rabbit mAb recognizes endogenous levels of total GM-CSF Receptor α protein. This antibody does not cross-react with GM-CSF Receptor β protein. Based on the antigen sequence, this antibody is predicted to recognize multiple isoforms of GM-CSF Receptor α protein, including secreted isoforms.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human GM-CSF Receptor α protein.
Background
Granulocyte-macrophage colony-stimulating factor receptor subunit alpha (GM-CSF Receptor α), also known as CSF2RA and CD116, is a type 1 transmembrane protein with low affinity for GM-CSF. GM-CSF Receptor α is primarily expressed on myeloid cells, including granulocytes, monocytes, macrophages, and dendritic cells (1). GM-CSF Receptor α forms a heterodimeric receptor complex with GM-CSF Receptor β, creating the high affinity GM-CSF receptor (2). Upon dimerization and ligand binding, the β subunit becomes phosphorylated and transduces signals via Jak/STAT and MAPK pathways for cell proliferation, differentiation, and survival (3,4). Oligomerization of the high affinity GM-CSF Receptor α/β complex is required for optimal intracellular signal transduction (5). Soluble GM-CSF Receptor α, either secreted or cleaved from the cell surface, can competitively bind GM-CSF, preventing membrane receptor signaling (1,6,7). Multiple isoforms of GM-CSF Receptor α have been described.
- Barreda, D.R. et al. (2004) Dev Comp Immunol 28, 509-54.
- Hansen, G. et al. (2008) Cell 134, 496-507.
- Doyle, S.E. and Gasson, J.C. (1998) Blood 92, 867-76.
- Lopez, A.F. et al. (2010) IUBMB Life 62, 509-18.
- McClure, B.J. et al. (2003) Blood 101, 1308-15.
- Raines, M.A. et al. (1991) Proc Natl Acad Sci U S A 88, 8203-7.
- Geijsen, N. et al. (2001) Cytokine Growth Factor Rev 12, 19-25.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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