GP130 Antibody #3732
Filter:
- WB
Western blot analysis of extracts from NIH/3T3, PC12, COS and HeLa cells, using GP130 Antibody.
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 130 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
GP130 Antibody detects endogenous levels of total GP130 protein independent of phosphorylation.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human GP130. Antibodies are purified by protein A and peptide affinity chromatography.
Background
GP130 is a signal-transducing subunit shared by the receptors for the interleukin-6 (IL-6) family of cytokines (1-3). The binding of a ligand to its receptor induces GP130 dimerization, leading to Jak tyrosine kinase activation and GP130 tyrosine phosphorylation. These events lead to the activation of multiple signal-transduction pathways, such as the Stat, Ras-MAPK, and PI3 kinase pathways, whose activation is controlled by distinct regions of GP130 (4-7).
- Taga, T. (1996) J. Neurochem. 67, 1-10.
- Taga, T. (1997) Ann. Med. 29, 63-72.
- Taga, T. and Kishimoto, T. (1997) Annu. Rev. Immunol. 15, 797-819.
- Heinrich, P.C. et al. (1998) Biochem. J. 334, 297-314.
- Ihle, J.N. et al. (1994) Trends Biochem. Sci. 19, 222-227.
- Kurth, I. et al. (1999) J. Immunol. 162, 1480-1487.
- Paonessa, G. et al. (1995) EMBO J. 14, 1942-1951.
Pathways
Explore pathways related to this product.
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