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  3. Iba1/AIF-1 (E4O4W) XP® Mouse Chimeric mAb
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Iba1/AIF-1 (E4O4W) XP® Mouse Chimeric mAb #58410

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  • IF

    Supporting Data

    REACTIVITY H M R Hm Mk
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse Chimera IgG2a
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Hm-Hamster 
    • Mk-Monkey 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as Iba1/AIF-1 (E4O4W) XP® Rabbit mAb #17198.

    Product Usage Information

    Application Dilution
    Immunofluorescence (Frozen) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Iba1/AIF-1 (E4O4W) XP® Mouse Chimeric mAb recognizes endogenous levels of total Iba1/AIF-1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Hamster, Monkey

    Source / Purification

    This recombinant chimeric antibody is engineered from Iba1/AIF-1 (E4O4W) XP® Rabbit mAb #17198 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.


    The parent antibody, Iba1/AIF-1 (E4O4W) XP® Rabbit mAb #17198, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala139 of human Iba1/AIF-1 protein.

    Background

    Ionized calcium-binding adaptor molecule 1 (Iba1), also known as allograft inflammatory factor 1 (AIF-1), is an evolutionarily conserved cytoplasmic calcium binding protein containing a central pair of EF-hand calcium binding motifs (1,2). Iba1/AIF-1 was originally cloned from activated macrophages in human atherosclerotic allogenic heart grafts undergoing chronic transplant rejection as well as from rat monocytes (3,4). Its function is not very well understood, but Iba1/AIF-1 expression is upregulated in response to interferon-gamma and, therefore, could modulate macrophage-dependent immune response (3). As an F-actin-binding protein, Iba1/AIF-1 may function to remodel the actin cytoskeleton and contribute to morphological changes that correlate with various microglial/macrophage states (5). Iba1/AIF-1 is also uniquely expressed in cells of monocytic lineage and is, therefore, widely used as a marker for microglia/macrophages in the brain and other tissue (6).

    Database Links

    UniProt ID: P55008


    Entrez-Gene Id: 199


    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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