IRF Family Antibody Sampler Kit #57103
Western blot analysis of extracts from control HeLa cells (lane 1) or IRF-3 knockout HeLa cells (lane 2) using IRF-3 (D6I4C) XP® Rabbit mAb, #11904 (upper) or β-actin (13E5) Rabbit mAb, #4970 (lower). The absence of signal in the IRF-3 knockout HeLa cells confirms specificity of the antibody for IRF-3.
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| Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
|---|---|---|---|---|---|
| IRF-1 (D5E4) XP® Rabbit mAb #8478 | 20 µl | WB, IP, IHC, IF, F, C&R | H M R | 45-48 | Rabbit IgG |
| IRF-2 Antibody #4943 | 20 µl | WB, IP | H M R | 45 | Rabbit |
| IRF-3 (D6I4C) XP® Rabbit mAb #11904 | 20 µl | WB, IP, IF | H Mk | 50-55 | Rabbit IgG |
| IRF-4 (D9P5H) Rabbit mAb #15106 | 20 µl | WB, IP, ChIP | H M R | 51 | Rabbit IgG |
| IRF-5 Antibody #20261 | 20 µl | WB, IP | H M | 60 | Rabbit |
| IRF-6 Antibody #6948 | 20 µl | WB, IP | H | 58, 60 | Rabbit |
| IRF-7 (D2A1J) Rabbit mAb #13014 | 20 µl | WB | H | 65 | Rabbit IgG |
| IRF-8 (D20D8) Rabbit mAb #5628 | 20 µl | WB, IP, ChIP | H M | 50 | Rabbit IgG |
| IRF-9 (D2T8M) Rabbit mAb #76684 | 20 µl | WB, IP, IF, ChIP | H | 48 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | WB | Rab | Goat |
Product Information
Kit Usage Information
Protocols
- 4943: Western Blotting, Immunoprecipitation (Magnetic)
- 5628: Western Blotting, Immunoprecipitation (Agarose), ChIP Magnetic, Chromatin IP-seq
- 6948: Western Blotting, Immunoprecipitation (Magnetic)
- 7074: Western Blotting
- 8478: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Leica® Bond™), Immunohistochemistry (Paraffin), Immunofluorescence, Flow, CUT&RUN Assay
- 11904: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence
- 13014: Western Blotting
- 15106: Western Blotting, Immunoprecipitation (Agarose), ChIP Magnetic
- 20261: Western Blotting, Immunoprecipitation (Magnetic)
- 76684: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence, ChIP Magnetic
Product Description
The IRF Family Antibody Sampler Kit contains reagents to examine the total protein levels of various IRF isoforms. The kit contains enough primary and secondary antibodies to perform two western blot experiments with each primary antibody.
Background
Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).
Pathways
Explore pathways related to this product.
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