IRG1 Antibody #17805
Filter:
- WB
Western blot analysis of extracts from RAW 264.7 cells, untreated (-) or treated with LPS #14011 (5 μg/ml, 6 hr; +), using IRG1 Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- M-Mouse
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
IRG1 Antibody recognizes endogenous levels of total IRG1 protein.
Species Reactivity:
Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse IRG1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
IRG1 (Immune-responsive gene 1) is one of the most up-regulated genes in macrophages under proinflammatory conditions (1). It is also highly expressed in the pregnant uterus during implantation (2,3). IRG1 is a cis-aconitate decarboxylase that produces itaconic acid by decarboxylating cis-aconitic acid, an intermediate of the tricarboxylic acid cycle (4). Itaconic acid is an endogenous inhibitor of succinate dehydrogenase, linking macrophage metabolic rewiring and regulation of inflammation (5,6).
- Lee, C.G. et al. (1995) Immunogenetics 41, 263-70.
- Chen, B. et al. (2003) Mol Endocrinol 17, 2340-54.
- Cheon, Y.P. et al. (2003) Endocrinology 144, 5623-30.
- Michelucci, A. et al. (2013) Proc Natl Acad Sci U S A 110, 7820-5.
- Lampropoulou, V. et al. (2016) Cell Metab 24, 158-66.
- Cordes, T. et al. (2016) J Biol Chem 291, 14274-84.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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