Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Ki-67 (D3B5) Mouse Chimeric mAb #71098
Filter:
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse Chimera IgG2a |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Description
This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as Ki-67 (D3B5) Rabbit mAb #9129.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Ki-67 (D3B5) Mouse Chimeric mAb recognizes endogenous levels of total Ki-67 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
This recombinant chimeric antibody is engineered from Ki-67 (D3B5) Rabbit mAb #9129 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.
The parent antibody, Ki-67 (D3B5) Rabbit mAb #9129, is produced by immunizing animals with a recombinant protein fragment corresponding to residues near the central region of human Ki-67 protein.
The parent antibody, Ki-67 (D3B5) Rabbit mAb #9129, is produced by immunizing animals with a recombinant protein fragment corresponding to residues near the central region of human Ki-67 protein.
Background
Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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