Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
MMP-9 (E7N3Y) Rabbit mAb #24317
Filter:
- WB
Western blot analysis of extracts from various tissues using MMP-9 (E7N3Y) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of MMP-9 protein in mouse liver tissue is consistent with the predicted expression pattern.
Supporting Data
| REACTIVITY | M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 84, 92 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- M-Mouse
- R-Rat
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
MMP-9 (E7N3Y) Rabbit mAb recognizes endogenous levels of total MMP-9 protein. This antibody detects both mature MMP-9 (84 kDa) and proMMP-9 (full-length 92 kDa).
Species Reactivity:
Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to the amino terminus of mouse MMP-9 protein.
Background
The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors, and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7, and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis, and apoptosis (2-4). Research studies have shown that MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full-length protein (5).
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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