Neutrophil Elastase Antibody (Rodent Reactive) #44030
Filter:
- WB
Western blot analysis of extracts from 32D Clone 3 cells, untreated (-) or treated with Human Granulocyte Colony Stimulating Factor (hG-CSF) #8930 (100 ng/mL, 72 hr; +), mouse bone marrow, and NIH/3T3 cells using Neutrophil Elastase Antibody (Rodent Reactive) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Supporting Data
| REACTIVITY | M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 28 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- M-Mouse
- R-Rat
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Neutrophil Elastase Antibody (Rodent Reactive) recognizes endogenous levels of total mouse and rat neutrophil elastase protein. This antibody does not cross-react with endogenously expressed human neutrophil elastase protein.
Species Reactivity:
Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val100 of mouse neutrophil elastase protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Neutrophil elastase is a hematopoietic serine protease that belongs to the chymotrypsin superfamily and plays a critical role in the innate immune function of mature neutrophils and monocytes (1,2). Neutrophil elastase is actively synthesized as an inactive zymogen in myelocytic precursor cells of the bone marrow, which then undergoes activation by limited proteolysis and sorting to primary (azurophil) storage granules of mature neutrophil granulocytes for regulated release (3,4). Research studies have shown that neutrophils play a significant role in mediating the inflammatory response through the release of neutrophil elastase, which activates pro-inflammatory cytokines and degrades components of the extracellular matrix and Gram-negative bacteria (5). Mutations in the gene encoding neutrophil elastase, ELA2, have been implicated in hematological diseases such as cyclic and severe congenital neutropenia, which is characterized by defects in promyelocyte maturation (6,7).
- Belaaouaj, A. et al. (2000) Science 289, 1185-8.
- Tkalcevic, J. et al. (2000) Immunity 12, 201-10.
- Gullberg, U. et al. (1999) J Immunol Methods 232, 201-10.
- Takahashi, H. et al. (1988) J Biol Chem 263, 2543-7.
- Adkison, A.M. et al. (2002) J Clin Invest 109, 363-71.
- Horwitz, M. et al. (1999) Nat Genet 23, 433-6.
- Bellanné-Chantelot, C. et al. (2004) Blood 103, 4119-25.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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