NSUN2 Antibody #44056
Filter:
- WB
- IP
Western blot analysis of extracts from 293T cells, either transfected with non-targeting siRNA (293T siNT) or siRNA targeting NSUN2 (293T siNSUN2), and various cell lines using NSUN2 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
NSUN2 Antibody recognizes endogenous levels of total NSUN2 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val690 of human NSUN2 protein. Antibodies are purified by peptide affinity chromatography.
Background
Chemical modifications of RNA regulate many cellular processes. One particular RNA modification, 5-methylcytosine (5-mC), regulates ribosome assembly, translation, and RNA stability (1). In eukaryotes, this modification is added to RNA by the DNA methyltransferase homologue 2 protein (DNMT2; also known as TRDMT1), and also by members of the NOL1/NOP2/SUN domain (NSUN) family of proteins. NSUN proteins are putative S-adenosylmethionine (SAM)-dependent methyltransferases that carry out their enzymatic activity by utilizing two cysteine residues in their active sites (2). There are currently seven known members of this family, consisting of NOP2 (NSUN1) and NSUN2-7.
NSUN2 is an 86 kDa member of the NSUN family that is predominantly localized to the nucleus and methylates a variety of tRNAs and other RNA substrates (3). NSUN2 has been found to play a role in the methylation of pre-tRNALeu, tRNAGly, tRNAVal, tRNALeu, and tRNAAsp (1,4,5). It has also been found to methylate mRNAs and several ncRNAs, such as vtRNAs (6-8). Elevated NSUN2 protein levels have been reported in multiple types of human cancers, including breast, prostate, kidney, bladder, and liver, among others (9). The methyltransferase activity of NSUN2 is reduced upon Aurora B kinase-mediated phosphorylation at Ser139 (10), and there is potential for NSUN2 to be used as a therapeutic target or diagnostic marker for Aurora B linked cancers (9).
NSUN2 is an 86 kDa member of the NSUN family that is predominantly localized to the nucleus and methylates a variety of tRNAs and other RNA substrates (3). NSUN2 has been found to play a role in the methylation of pre-tRNALeu, tRNAGly, tRNAVal, tRNALeu, and tRNAAsp (1,4,5). It has also been found to methylate mRNAs and several ncRNAs, such as vtRNAs (6-8). Elevated NSUN2 protein levels have been reported in multiple types of human cancers, including breast, prostate, kidney, bladder, and liver, among others (9). The methyltransferase activity of NSUN2 is reduced upon Aurora B kinase-mediated phosphorylation at Ser139 (10), and there is potential for NSUN2 to be used as a therapeutic target or diagnostic marker for Aurora B linked cancers (9).
- Trixl, L. and Lusser, A. (2019) Wiley Interdiscip Rev RNA 10, e1510.
- Bohnsack, K.E. et al. (2019) Genes (Basel) 10, pii: E102. doi: 10.3390/genes10020102.
- Bourgeois, G. et al. (2015) PLoS One 10, e0133321.
- Brzezicha, B. et al. (2006) Nucleic Acids Res 34, 6034-43.
- Tuorto, F. et al. (2012) Nat Struct Mol Biol 19, 900-5.
- Tang, H. et al. (2015) Aging (Albany NY) 7, 1143-58.
- Xing, J. et al. (2015) Mol Cell Biol 35, 4043-52.
- Hussain, S. et al. (2013) Cell Rep 4, 255-61.
- Okamoto, M. et al. (2012) DNA Cell Biol 31, 660-71.
- Sakita-Suto, S. et al. (2007) Mol Biol Cell 18, 1107-17.
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