Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Olig2 (E6G6Q) XP® Feline Chimeric mAb #90646
Filter:
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Feline Chimera IgG1 |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Description
This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as Olig2 (E6G6Q) XP® Rabbit mAb #65915.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Olig2 (E6G6Q) XP® Feline Chimeric mAb recognizes endogenous levels of total Olig2 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
This recombinant chimeric antibody is engineered from Olig2 (E6G6Q) XP® Rabbit mAb #65915 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.
The parent antibody, Olig2 (E6G6Q) XP® Rabbit mAb #65915, is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human Olig2 protein.
The parent antibody, Olig2 (E6G6Q) XP® Rabbit mAb #65915, is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human Olig2 protein.
Background
Olig2 is a basic helix–loop–helix (bHLH) transcription factor necessary for oligodendrocyte and motor neuron differentiation and development in the central nervous system (1,2). Olig2 has been shown to play a role in Purkinje cell specification in the early cerebellum (3). Olig2 has also been shown to be a useful marker for primary and mature oligodendrocytes and is universally expressed in diffuse gliomas (4,5).
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For Research Use Only. Not for Use in Diagnostic Procedures.
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