Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Parvalbumin (E8N2U) XP® Horse Chimeric mAb #64370
Filter:
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Horse Chimera IgG4 |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Description
This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as Parvalbumin (E8N2U) XP® Rabbit mAb #80561.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Parvalbumin (E8N2U) XP® Horse Chimeric mAb recognizes endogenous levels of total parvalbumin protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
This recombinant chimeric antibody is engineered from Parvalbumin (E8N2U) XP® Rabbit mAb #80561 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a horse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.
The parent antibody, Parvalbumin (E8N2U) XP® Rabbit mAb #80561, is produced by immunizing animals with a recombinant protein specific to the amino terminus of human parvalbumin protein.
The parent antibody, Parvalbumin (E8N2U) XP® Rabbit mAb #80561, is produced by immunizing animals with a recombinant protein specific to the amino terminus of human parvalbumin protein.
Background
Parvalbumin (PV, PVALB) is a member of the EF-hand family of calcium-binding proteins, containing EF-hand domains with a high affinity for calcium and magnesium (1). Parvalbumin is expressed in both the soma and in processes of specific subclasses of GABAergic interneurons. Parvalbumin is a slow calcium buffer, meaning it accelerates the initial phase of decay of calcium after an action potential, which likely inhibits facilitation and is thought to regulate short-term synaptic plasticity (2,3). Altered function of PV positive interneurons has been implicated in many neurological diseases, such as Alzheimer’s disease, autism spectrum disorder, schizophrenia, and bipolar disorder (4).
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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