Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Pellino-1 (D2Z4F) Rabbit mAb #31474
Filter:
- WB
Western blot analysis of extracts from various cell lines using Pellino-1 (D2Z4F) Rabbit mAb.
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 47 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Pellino-1 (D2Z4F) Rabbit mAb recognizes endogenous levels of total Pellino-1 protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala403 of human Pellino-1 protein.
Background
Pellino-1 (PELI1) is an E3 ubiquitin ligase that regulates activation of various immune pathways in different contexts and cell types (1). Pellino-1 controls NFκB activation downstream of the interleukin-1 receptor and TLRs through association with and ubiquitination of IRAK1 (1-4). Pellino-1 was also demonstrated to regulate the NFκB pathway in T cells by targeting c-Rel for degradation, thereby enabling suppression by regulatory T cells, maintaining peripheral tolerance, and preventing autoimmunity in mice (5). Finally, in experimental autoimmune encephalomyelitis (EAE), the mouse model of multiple sclerosis, Pellino-1 contributes to inflammation by promoting degradation of Traf3 leading to induction of chemokines and proinflammatory cytokines (6).
Pathways
Explore pathways related to this product.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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