Phospho-Jak1 (Tyr1034/1035) Antibody #3331
Filter:
- WB
Western blot analysis of extracts from various cell lines, untreated, or treated with IL-2 (#68966, 10 ng/mL, 15 min), IL-3 (#98018, 10 ng/mL, 5 min), IL-4 (#8919, 10 ng/mL, 10 min), or IFNa (#36000, 10 ng/mL, 15 min); using Phospho-Jak1 (Tyr1034/1035) Antibody #3331 (upper), Jak1 Mouse mAb #50996 (middle), and β-Actin (8H10D10) Mouse mAb #3700 (lower).
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 130 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Phospho-Jak1 (Tyr1034/1035) Antibody detects endogenous levels of Jak1 only when phosphorylated at tyrosines 1034/1035. Human Jak1 residues Tyr1034 and Tyr1035 historically have been referenced as Tyr1022 and Tyr1023. This antibody may cross-react with phospho-Jak2.
Species Reactivity:
Human, Mouse
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Rat, Bovine
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1034/1035 of human Jak1. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.
The tyrosine residues 1022/1023 of Jak1 in the putative activation loop are the homologous tyrosine residues 1054/1055 in Tyk2, which are important in the regulation of Tyk2 kinase activity (6).
(This product is sold under license from Chemicon, Inc., U.S. Patent No. 5,658,791.)
The tyrosine residues 1022/1023 of Jak1 in the putative activation loop are the homologous tyrosine residues 1054/1055 in Tyk2, which are important in the regulation of Tyk2 kinase activity (6).
(This product is sold under license from Chemicon, Inc., U.S. Patent No. 5,658,791.)
- Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
- Darnell, J.E. (1997) Science 277, 1630-5.
- VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
- Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
- Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.
- Gauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-20500.
Pathways
Explore pathways related to this product.
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For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Jak antibodies produced under license (granting certain rights including those under U.S. Patent No. 5,658,791) from Chemicon International, Inc.
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