Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Phospho-STING (Ser365) (D1C4T) Rabbit mAb #62912
Filter:
- IF
Confocal immunofluorescent analysis of Raw 264.7 cells, transfected with poly(dA:dT) (5 ug/ml, 3 hr; left) or mock transfected (right), using Phospho-STING (Ser365) (D1C4T) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- M-Mouse
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #51865.
For a carrier free (BSA and azide free) version of this product see product #51865.
Protocol
Specificity / Sensitivity
Phospho-STING (Ser365) (D1C4T) Rabbit mAb recognizes endogenous levels of STING protein only when phosphorylated at Ser365.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser365 of mouse STING protein.
Background
Stimulator of interferon genes (STING, TMEM173, MITA) is a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1,2). STING is a ubiquitously expressed protein found predominantly in the ER (1). The enzyme cGAMP synthase (cGAS) produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (3,4). cGAMP binds and activates STING (3,4). In addition, detection of cytoplasmic DNA by nucleic acid sensors, including DDX41 or IFI16, results in STING activation (5,6). Following activation, STING translocates with TBK1 to perinuclear endosomes (7). The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes (1,2,7).
Following binding of cyclic dinucleotides, STING is phosphorylated by TBK1 at Ser366 (Ser365 in mouse), leading to IRF-3 activation and type I interferon upregulation (8).
Following binding of cyclic dinucleotides, STING is phosphorylated by TBK1 at Ser366 (Ser365 in mouse), leading to IRF-3 activation and type I interferon upregulation (8).
- Ishikawa, H. and Barber, G.N. (2008) Nature 455, 674-8.
- Zhong, B. et al. (2008) Immunity 29, 538-50.
- Sun, L. et al. (2013) Science 339, 786-91.
- Wu, J. et al. (2013) Science 339, 826-30.
- Zhang, Z. et al. (2011) Nat Immunol 12, 959-65.
- Unterholzner, L. et al. (2010) Nat Immunol 11, 997-1004.
- Ishikawa, H. et al. (2009) Nature 461, 788-92.
- Liu, S. et al. (2015) Science 347, aaa2630.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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