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Phospho-STING (Ser366) (D8K6H) Rabbit mAb #40818

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  • IF
  • F
Confocal immunofluorescent analysis of THP-1 cells differentiated with TPA (12-O-Tetradecanolphorbol) #4174 (80 nM, 16 hr) and then transfected with poly(dA:dT) (5 μg/mL, 3 hr; left) or mock transfected (right) using Phospho-STING (Ser366) (D8K6H) Rabbit mAb (green), DyLight™ 554 Phalloidin #13054 (red), and DAPI #4083 (blue).

To Purchase # 40818

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Rabbit IgG
Application Key:
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products
  • Conjugates

Product Information

Product Usage Information

Application Dilution
Immunofluorescence (Immunocytochemistry) 1:800 - 1:1600
Flow Cytometry (Fixed/Permeabilized) 1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #99309.

Protocol

Specificity / Sensitivity

Phospho-STING (Ser366) (D8K6H) Rabbit mAb recognizes endogenous levels of STING protein only when phosphorylated at Ser366.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser366 of human STING protein.

Background

Stimulator of interferon genes (STING, TMEM173, MITA) is a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1,2). STING is a ubiquitously expressed protein found predominantly in the ER (1). The enzyme cGAMP synthase (cGAS) produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (3,4). cGAMP binds and activates STING (3,4). In addition, detection of cytoplasmic DNA by nucleic acid sensors, including DDX41 or IFI16, results in STING activation (5,6). Following activation, STING translocates with TBK1 to perinuclear endosomes (7). The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes (1,2,7).

Following binding of cyclic dinucleotides, STING is phosphorylated by TBK1 at Ser366 (Ser365 in mouse), leading to IRF-3 activation and type I interferon upregulation (8).
For Research Use Only. Not for Use in Diagnostic Procedures.
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