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SF2/ASF (D6S7V) Rabbit mAb #14908

Filter:
  • WB
Western Blotting Image 1: SF2/ASF (D6S7V) Rabbit mAb
Western blot analysis of extracts from various cell lines using SF2/ASF (D6S7V) Rabbit mAb.

To Purchase # 14908

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 33
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

SF2/ASF (D6S7V) Rabbit mAb recognizes endogenous levels of total SF2/ASF protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly53 of human SF2/ASF protein.

Background

SF2/ASF is a member of the Ser-Arg-rich (SR) protein family of highly conserved nuclear phosphoproteins involved in pre-mRNA splicing (1). Besides its role in nuclear pre-mRNA splicing, SF2/ASF has been shown to shuttle between the nucleus and cytoplasm, suggesting additional roles in mRNA transport and cytoplasmic events (2). SF2/ASF associates with translating ribosomes and stimulates translation (3). It also activates translation initiation by suppressing the activity of 4E-BP1, which is mediated by SF2/ASF association with mTOR and the phosphatase PP2A (4). More recent studies have demonstrated a role for SF2/ASF in microRNA processing (5).
For Research Use Only. Not for Use in Diagnostic Procedures.
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