Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SLC25A39 (F2F6O) Rabbit mAb #33871
Filter:
- WB
Western blot analysis of extracts from K-562 cells, untreated (-) or treated with L-Buthionine-sulfoximine (BSO) (10 μM, 48 hr; +), using SLC25A39 (F2F6O) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). SLC25A39 is induced by BSO treatment as expected.
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 36 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SLC25A39 (F2F6O) Rabbit mAb recognizes endogenous levels of total SLC25A39 protein. A band of unknown origin is detected at ~90 kDa.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SLC25A39 protein.
Background
Members of the solute carrier 25 (SLC25) family transport molecules over the mitochondrial inner membrane (1,2). Mutations in these carriers often result in rare but severe pathologies (3). SLC25A39 and its paralogue SLC25A40 were found to be essential for mitochondrial import of glutathione, providing protection against oxidative stress and mitochondrial dysfunction (4,5). Changes in SLC25A39 and SLC25A40 expression were found in cholestasis-associated renal injury and LPS-induced inflammation (6).
- Ruprecht, J.J. and Kunji, E.R.S. (2021) Annu Rev Biochem 90, 535-558.
- Kunji, E.R.S. et al. (2020) Physiology (Bethesda) 35, 302-327.
- Palmieri, F. et al. (2020) Biomolecules 10, 655. doi: 10.3390/biom10040655.
- Wang, Y. et al. (2021) Nature 599, 136-140.
- Shi, X. et al. (2022) Nat Commun 13, 2483.
- Kawase, A. et al. (2022) Int J Mol Sci 23, 8573. doi: 10.3390/ijms23158573.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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