Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
VISTA (D1L2G™) XP® Rabbit mAb #64953
Filter:
- WB
- IHC
- F
Western blot analysis of extracts from OVKATE, DU 145, and MCF7 cells using VISTA ((D1L2G™) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45-65 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
- Related Products
- Conjugates
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:100 - 1:400 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier-free (BSA and azide free) version of this product see product #82119.
For a carrier-free (BSA and azide free) version of this product see product #82119.
Protocol
Specificity / Sensitivity
VISTA (D1L2G™) XP® Rabbit mAb recognizes endogenous levels of total VISTA protein. Non-specific staining was observed in kidney and prostate epithelium by immunohistochemistry.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human VISTA protein.
Background
VISTA (V-Domain Ig Suppressor of T Cell Activation) is a negative checkpoint control protein that regulates T cell activation and immune responses. VISTA, which contains a single Ig-like V-type domain, a transmembrane domain, and an intracellular domain, has sequence similarity to both the B7 and CD28 family members. Although primarily expressed by myeloid cells, VISTA is also expressed by CD4+, CD8+, and FoxP3+ T-cells. Thus, VISTA is described as both a ligand and a receptor (1-3). Blocking VISTA induces T-cell activation and proliferation, and potentiates disease severity in the EAE model (1). Furthermore, genetic deletion of VISTA in mice leads to spontaneous T-cell activation and chronic inflammation (4,5). In mouse models of cancer, neutralization of VISTA enhances T-cell proliferation and effector function and increases tumor infiltration, suggesting VISTA blockade could be an effective strategy for tumor immunotherapy (6,7).
- Wang, L. et al. (2011) J Exp Med 208, 577-92.
- Flies, D.B. et al. (2011) J Immunol 187, 1537-41.
- Lines, J.L. et al. (2014) Cancer Res 74, 1924-32.
- Wang, L. et al. (2014) Proc Natl Acad Sci U S A 111, 14846-51.
- Liu, J. et al. (2015) Proc Natl Acad Sci U S A 112, 6682-7.
- Le Mercier, I. et al. (2014) Cancer Res 74, 1933-44.
- Lines, J.L. et al. (2014) Cancer Immunol Res 2, 510-7.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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