Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127
Filter:
- WB
Immunoprecipitation of PRAS40 from HeLa cells using PRAS40 (D23C7) XP® Rabbit mAb #2691 (lane 2). Lane 1 contains input control lysate. All lanes are probed with PRAS40 (D23C7) Rabbit mAb #2691 as the primary antibody. Secondary antibodies include Anti-rabbit IgG, HRP-linked Antibody #7074 (panel A) and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 (panel B).
Supporting Data
| REACTIVITY | Rab |
| SENSITIVITY | |
| MW (kDa) | |
| Source/Isotype | Mouse |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- Rab-Rabbit
- Related Products
Product Information
Product Description
Affinity purified mouse anti-rabbit IgG (Conformation Specific) HRP Conjugate antibody.
Product Usage Information
Recommended Antibody Dilution:
1:2000
Storage
Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Protocol
Specificity / Sensitivity
Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) recognizes the native rabbit IgG. This antibody may cross react with denatured IgG at high concentrations.
Species Reactivity:
Rabbit
Source / Purification
Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.
Background
Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb only reacts with native IgG and does not bind to the denatured and reduced rabbit IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb as a secondary antibody will eliminate this problem.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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