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α-Smooth Muscle Actin (D4K9N) & CO-0024-647 SignalStar Oligo-Antibody Pair #63902

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  • IHC
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma using α-Smooth Muscle Actin (D4K9N) & CO-0024-750 SignalStar™ Oligo-Antibody Pair #29280 (cyan), CD31 (PECAM-1) (89C2) & CO-0028-488 SignalStar™ Oligo-Antibody Pair #83823 (green), CD68 (D4B9C) & CO-0007-594 SignalStar™ Oligo-Antibody Pair #77318 (yellow), and Pan-Keratin (C11) & CO-0003-647 SignalStar™ Oligo-Antibody Pair #16373 (red). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.

Order Information # 63902

This product is not sold separately. Please see the SignalStar® Multiplex IHC Panel Builder Tool for ordering information.

  • Product Includes
  • Related Products
Product IncludesVolumeReactivityIsotype
α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (SignalStar™ Conjugate 0024) #7115950 µlH MRabbit IgG
Complementary Oligo (CO-0024-647) #6586422 µl

Product Information

Product Usage Information

Application Dilution
SignalStar™ Leica Bond 1:50 - 1:200
SignalStar™ Manual 1:50 - 1:200

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Product Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Protocol

Specificity / Sensitivity

α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (SignalStar™ Conjugate 0024) recognizes endogenous levels of total α-smooth muscle protein.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human α-smooth muscle actin protein.

Background

Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility (1). The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells (1).

Pathways

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For Research Use Only. Not for Use in Diagnostic Procedures.
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